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May 05, 2024
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BINF 6350L - Biotechnology and Genomics Hands on Laboratory
Credit Hours: (0)
Hands-on experience producing sequencing templates and libraries. Introduction to the practical skills needed to carry out a series of experiments that result in sequence data. The unifying concept is to characterize allelic variants of selected genes from related organisms. Students purify nucleic acid and then produce a selected subset of each genome using PCR. Quality control via spectroscopy, gel electrophoresis and quantitative PCR are performed. Sequencing libraries are produced and run on the Ion Torrent PGM and the ABI 3130 Genetic Analyzer. The CLCbio Genomics Workbench software for assessing data quality and identifying polymorphisms will be utilized. Students expected to keep laboratory notebooks that allow all aspects of experiments to be reconstructed.
Corequisite(s): BINF 6350 .
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